Get Started
Aim : To identify and characterize reducing sugars.
Materials Required : The Test Solution, Phenylhydrazine-hydrochloride, Glacial Acetic acid, Sodium Acetate
Methodology An aqueous solution of around 5 mL of carbohydrate sample is taken in a clean and dry boiling tube.
To the above solution, 10 drops of glacial acetic acid and approx 5 drops of phenylhydrazine are added.
The solution is shaken well and the mixture is then kept in boiling water (water bath).
Now, time is noted at the various time interval for the formation of osazone.
After the formation of osazone, the ppt is washed with water, it should be recrystallized from hot water for better purity and for further analyzing purposes.
Then, it can be observed under a microscope for clear morphological structure.
Result Osazone formation is a chemical reaction in which reducing sugars
react with phenylhydrazine, yielding osazones as crystalline derivatives, commonly used for sugar identification
and characterization.